Programme

09:00-10:30: Session 2a – Models, biomarkers and assays for endocrine disruption
Chairs: Christian Pellevoisin (Urbilateria & Vitroscreen, France) and Prof Arno Gutleb (Invitrolize & Luxembourg Institute of Science and Technology LIST, Luxembourg, Luxembourg)

• Daniela Lange – Evaluation of in vitro and in silico new alternative methods for the identification of potential endocrine effects
• Christian Pellevoisin – A 3d model applicable to reproductive toxicology
• Kim Heikamp – Human based brain barrier models for studying thyroid hormone transport
• Petra Mikušová – The identification of thyroid hormone-disrupting chemicals from complex environmental mixtures using pull-down assay coupled with non-target analysis
• Runze Liu – Novel in vitro assay for thyroperoxidase inhibition – comparison of different models and detection methods

09:00-10:30: Session 2b – Challenges in cosmetics safety
Chairs: Dr Bambou Tan (L’Oréal, Paris, France) and Erin Hill (ICCS, New York, USA)

• Andy Forreryd – Improved confidence of quantitative sensitizing potency assessment for point of departure using Gardskin dose-response
• Fleur Tourneix  – Deriving a continous point of departure for skin sensitization assessment using a bayesian network model  
• Johanna Ebmeyer – A novel 3d Nrf2-are reporter epidermis model for skin sensitization testing

11:00-13:00: Session 3a – Human Cells as New Approach Methodologies for Immunotoxicity Testing
Chairs: Dr Victor J. Johnson (Burleson Research Technologies, Inc., Morrisville, NC, USA) and Prof Emanuela Corsini (Università degli Studi di Milano, Milan Italy)

• Victor J. Johnson  – Building a comprehensive toolbox for Immunotoxicology safety assessment using human whole blood.
• Emanuela Corsini – Using in vitro human immune system models to understand the immunotoxicity risks associated with PFAS exposure
• Fenna Sillé – A Path Forward: Current and Future Perspectives of Alternatives to Developmental Immunotoxicity Testing
• Norbert E. Kaminski – Human cord blood derived CD34+ hematopoietic stem cells as an in vitro model for investigating developmental immunotoxicity
• Mélanie Mourot-Bousquenaud – In vitro assessment of the sensitizing potential of bisphenol A substitutes

11:00-13:00: Session 3b – Organ-on-a-chip & Microphysiology systems
Chairs: Dr Benoît Maisonneuve (NETRI, Lyon, France) and Dr Erika Ferrari (BiomimX, Milano, Italy)

• Erika Ferrari – Evaluating the potential of novel physiologic-like liver-on-chip models for in vitro hepatotoxicity screening
• Kaushal Joshi – A pilot study to evaluate the kinetics, metabolism, and toxicity of a fragrance material (coumarin) in an in vitro three-organ integrated microphysiological system (MPS) model
• Maria-Jose Ruiz – Cytotoxic effects induced by individual and combined exposure to sterigmatocystin, ochratoxin A, and patulin on sh-sy5y spheroids
• Marie-Gabrielle Zurich – Novel strategy to assess the neurotoxicity of organic solvents such as glycol ethers: combining in vitro and in silico methods with human-controlled exposure experiments
• Ovidiu Novac – A sensitive and robust human liver microphysiological system for assessing drug-induced liver injury
• Eileen Hallscheidt – Oral absorption and intestinal first-pass metabolism of pesticides using an in vitro human gut-model

13:00-14:00: Lunch break, visiting exhibition area & poster viewing

13:00-14:00: ASPIS Training Academy session – Organised by Early Career scientists of ONTOX, RiskHunt3Rs and PrecisionTox

14:00-16:00: Session 4a – Computational toxicology – in silico modelling, read-across, artificial intelligence
Chairs: Prof Thomas Hartung (Johns Hopkins Bloomberg School of Public Health and the Whiting School of Engineering, Baltimore, USA) and Emily Reinke (Inotiv, Research Triangle Park, NC, United States)

• Emily Reinke – Case studies for the sara-ice defined approach for skin sensitization – application to different chemistries
• Fresnais Louison – Metabolic mechanisms of action modelling to sustain safety evaluation of cosmetic ingredients
• Prachi Pradeep – New approach methods for regulatory risk assessment of azo dyes in textiles
• Simon Perera del Rosario – Anthrodrugs-tox: personalized toxicology through population genetics
• Vijay Gombar – Integration of multi-domain data and interpretable in-silico models for computational toxicology

14:00-16:00: Session 4b – Local toxicity testing (safety and efficacy)
Chairs: Dr Helena Kandarova (CEM SAS, Bratislava, Slovakia) and Dr Eugene Choi (OECD, Paris, France)

• Marco Pelin – Adoption of OECD TG 439 and 431 for the assessment of skin irritation and corrosion properties of 2D nanomaterials
• Maria Laura Gutierrez – Eye damage reversibility in an in vitro model of bovine cornea to replace the Draize test completely
• Rodrigo Azevedo Loiola – The Interleukin-18 is an in vitro biomarker for the prediction of photosensitization in reconstructed human epidermis: the development of photosensil-18 method for the safety assessment of raw materials and finished products
• Vérane Bard – Toxicity of polylactic acid and polyethylene terephthalate nanoplastics, aged in environmental conditions, on human intestinal cells

16:30-17:30: Early Stage Researcher Session 1
Chairs: Marketa Dvořáková (NIPH Prague, Praha, Czech Republic) and Mohamed Fathi Abdallah Abdelmohsen (Ghent University, Ghent, Belgium)

• Arunima Sengupta – A new lung-on-chip inhalation platform for toxicity and therapy assessment
• Eliška Řehůřková – Utilizing human induced pluripotent stem cells to enhance in vitro leydig cell models for reproductive health research
• Eliška Sychrová – Male reproductive toxicity of real-life PFAS mixtures: an AOP-based investigation
• Giulia De Negri Atanasio – Evaluation of physiological repeated exposure of aluminium in a 3d intestinal tissue model

16:30-17:30: Industry session – In vitro inhalation toxicity and future trends for Fiber Safety assessment
Chairs: Prof Arno Gutleb (Invitrolize & Luxembourg Institute of Science and Technology LIST, Luxembourg, Luxembourg) and Dr Samuel Constant (Epithelix, Plan-les-Ouates, Switzerland)

• Amy Madl – Toxicology of synthetic vitreous fibers (svfs): Historical overview and outlook for next generation new approach methods (NAMs)
• Annette Bitsch – The problem of lung overload in animal studies
• Samuel Constant – Advanced immunocompetent in vitro primary human lung models for point-of-contact toxicity evaluation of man-made vitreous fibres (mmvf)

17:30-18:30: Early Stage Researcher Session 2
Chairs: Marketa Dvořáková (NIPH Prague, Praha, Czech Republic) and Mohamed Fathi Abdallah Abdelmohsen (Ghent University, Ghent, Belgium

• Ignacio Risueño -A novel way to measure cell viability with electrochemical sensing for in vitro skin testing
• Julia Nöth -Analysis of vascular disruptors in zebrafish embryos – an endpoint of developmental toxicity
• Krupansh Desai -In vitro biocompatibility assessment of engineered living materials: a high throughput strategy
• Laurie Perdigon -Successful generation of three-dimensional human precision-cut lung slices for pulmonary fibrosis modeling
• Liyi Tan – Toxic effects of metal oxide nanoparticles on healthy and psoriatic-like human epidermal keratinocytes
• Peter Pôbiš – Development of sensitive in vitro protocols for biocompatibility testing of medical devices and pharmaceuticals intended for contact with eyes:

17:30-19:30: Peer Review for Validation Studies – building confidence and transparency into a new validation paradigm
Chairs: Amanda Ulrey (IIVS, Gaithersburg, USA) and João Barroso (JRC, Ispra, Italy)

• João Barroso -The art of validation and peer review: evolving practices while ensuring scientific confidence
• G. Frank Gerberick – Ensuring transparency and rigor in the peer review of new approach methods for skin sensitization assessment
• Hervé Groux – Peer review for validation studies – building confidence and transparency into a new validation paradigm- the test developer perspective
• Sebastian Hofmann – Comprehensive reporting of validation studies starts at inception and ends with peer review
• Amanda Ulrey – Assessing data integrity: a deep dive into data review in method validation
• Emily Reinke – Peer review for validation studies – building confidence and transparency into a new validation paradigm

09:00-10:30: Session 5a – PARC session – Partnership for the Assessment of Risks from Chemicals
Chairs: Prof Denis Sarigiannis (Aristotle University of Thessaloniki, Greece) and prof Philip Marx-Stoelting (German Federal Institute for Risk Assessment, Berlin, Germany)

• Denis Sarigiannis – Safe and Sustainable by Design
• Gilles Riviere – Hazard Assessment in PARC
• Sebastian Schmeisser – NGRAroute andd PARCopedia
• Dimitra Nikolopoulou – How PARC can contribute to promoting the regulatory uptake of NAMs

09:00-10:30: Session 5b – Knowledge sharing and education
Chairs: Dr. Bambou Tan (LOreal, Paris, France) and Erin Hill (ICCS, New York, USA)

• Bambou Xuezhu TAN – Storytelling skills for a more effective scientific communication
• Beta Montemayer – Skills for Next-Generation Safety Scientists in Cosmetics: Bridging Academic Expertise to Industry Relevance
• Erin Hill – International Collaboration for Cosmetics Safety (ICCS): Accelerating Global Adoption of Animal-Free Safety Science for Cosmetic Product and Ingredient Safety Assessment
• Philipp Paulitschke – New non-invasive, label-free monitoring approach for 2D and 3D cell culture
• Pascale Mora – Continuing education for next-generation risk assessment

11:00-13:00: Session 6a – Toxicokinetics and in vitro–in vivo extrapolation
Chairs: Jochem Louisse (European Food Safety Authority EFSA, Parma, Italy) and Andreas Schepky (Beiersdorf AG, Hamburg, Germany)

• Andreas Schepky – Relevance of Toxicokinetics and ADME methods in Quantifying Exposure for Next generation Risk Assessment
• Daniela Brenner – Toxicokinetic assessment of perfluorooctanoic acid and perfluorooctanesulfonic acid in a 2-dimensional in vitro liver model
• Elena Reale – Next-generation risk assessment for diterpene glycosides
• Evita Vandenbossche-Goddard – A workflow for true dose considerations of in vitro test systems which are used as part of next-generation risk assessment
• René Geci – High-throughput PBK modelling providing insights into ADME/TK in systemic toxicity
• Jochem Louisse – Towards a quantitative interpretation of in vitro DNT data using PBK-modelling based QIVIVE

11:00-13:00 Session 6b – In vitro systems to assess respiratory toxicity
Chairs: Prof Arno Gutleb (Invitrolize & Luxembourg Institute of Science and Technology LIST, Luxembourg, Luxembourg) and Robert Bedford

• Robert Bedford – An In Vitro Model to Mimic Immune and Vascular Responses Following Airway Inflammation in Humans
• Sabina Burla – Responses insided by AEROSIL® R 504 using ALISENS®
• Sonia Scarfì – Inflammatory and carcinogenic potential of mineral fibres assessed through a physiologically relevant 3d in vitro model of human alveolar tissue
• Xiao-Yann Huang – Development of an in vitro model of Dry Nose
• Sylvain Billet – Toxicity of mixtures: ethylbenzene and xylene have competitive effects in human lung cells
• Nienke Ruijter – The Sabyna in vitro hazard testing strategy for adverse effects upon inhalation of nanomaterials: ‘safe and sustainable by design’ made practical

13:00-14:00: Lunch break, visiting exhibition area & poster viewing

13:00-14:00: Session organised by The Centre for Animal-Free [Proefdiervrij] Biomedical Translation: Co-creating a life long training program

14:00-15:00 Session 7a- Bio-engineering, stem cells and disease models

Chairs: Dr Yasunari Kanda (National Institute of Health Sciences, Kawasaki, Japan) and Prof Anna Maria Bassi, (Universita degli Studi di Genova, Genova, Italy)

• Anna Maria Bassi – Differentiation of retinal organoids from human iPSC  for modelling  neurodegenerative diseases
• Yasunari Kanda – Prediction of developmental neurotoxicity using an integrated in silico and in vitro approach.
• Alexandra Gatzios– Genetic predisposition for anti-mash drug response:  a population-based in vitro study

14:00-15:00 Session 7b- Towards Liver models

Chairs: Mathieu Vinken (VUB, Brussels, Belgium) and Amer Jamalpoor (Toxys, Oegstgeest, The Netherlands)

• Anna Melina Steinbach – Towards in silico modelling of qAOPs for liver steatosis
• Heeseung Jo – A framework for a chemically agnostic model for liver injury as a stepping stone to link in vitro outcomes to organ-level adverse outcomes.
• Alkiviadis Stagkos-Georgiadis – Prioritisation of potential hepatotoxic co-formulants for chemical risk assessment using NAMs
• Anouk Verhoeven  – A stem cell-based in vitro test battery to predict liver steatotic potential of diverse chemicals

16:30-18:30:  Session 8a – Case studies for successful use and implementation of complex in vitro models
Chairs: Dr Andreas Stucki (PETA Science Consortium International e.V., Stuttgart, Germany) and Paul Carmichael (Unilever, UK and Wageningen University (WUR), Wageningen, The Netherlands

• Andreas O Stucki – The INSPIRE Initiative: Assessing respiratory toxicity of surfactants to human cell-based in vitro systems by pipetting and aerosol exposure
• Giel Hendricks – Validation and implementation of the toxtracker assay for mechanistic genotoxicity assessment
• Benoit Fischer – 2D and 3D human endothelial cell models to assess vascular toxicity
• Els Adriaens – Breaching the surface: in vitro Eye Hazard Assessment of surfactants based on an innovative Defined Approach
• Kristina Jochum – Comparative Case Studies on NAMs: A Step Towards Enhancing Specific Target Organ Toxicity Analysis
• Paul Carmichael -New Approach Methodologies (NAMs) for use in Next Generation Risk Assessment (NGRA) for Systemic Safety: A pragmatic approach to ‘validation’ by establishing protectiveness and utility

16:30-18:30 Session 8b – Complex chemical mixtures – how can NAMs contribute?
Chairs: Prof Anne Marie Vinggaard (DTU, Denemark) and Beate Escher (Helmholtz Centre for Environmental Research in Leipzig, Germany)

• Anne Marie Vinggaard – Using NAMs for predicting adverse effects of chemical mixtures on male reproductive health
• Beate Escher – In vitro assays for quantification of adverse effects of chemical mixtures extracted from human serum
• Wibke Busch – Combined effects of pesticide product ingredients in zebrafish and mixture effect predictions at the whole transcriptome scale
• Iva Sovadinová – Unraveling the impact of a real-life organochlorine mixture on male reproductive health: an AOP-driven approach combined with lipidomics
• Jiří Novák – Endocrine disruptive potencies of air and dust samples from different indoor environments

09:00-10:00: Session 9a – Developmental Toxicity and Developmental Neurotoxicity (DNT)
Chairs: Dr Yasunari Kanda (National Institute of Health Sciences, Kawasaki, Japan) and Dr Amer Jamalpoor (Toxys, Oegstgeest, The Netherlands)

• Robin Pronk – High-throughput screening of FDA-approved compounds on human brain organoids for safety assessment
• Jade Houghton – Evaluating the Reprotracker assay as a NAM for developmental and reproductive toxicity testing
• Matthew Burbank – Advancing the use of New Approach Methodologies for assessing teratogenicity: A tiered approach
• Chun-Wei Tung – Benchmarking in silico methods for identifying chemicals of developmental neurotoxicity concern

09:00-10:00: Session 9b – Regulatory considerations for complex NAMs
Chairs: Anne Gourmelon (OECD, Paris, France) and Dr Nathalie Alepee (L’Oreal, Paris, France)

• Nathalie Alepee – Quantifying the uncertainty of the new approach methodology U-SENS for skin sensitization assessment in a regulatory context
• Anne Gourmelon – The future of risk assessment at OECD
• Philip Marx-Stoelting – NAMs in chemical mixture risk assessment
• Raechel Puglisi – Survey results to identify nam use in agrochemical regulation

10:00-11:00: Session 10a – In vitro methods for safety testing of biopharmaceuticals/biotherapies/vaccines
Chairs: Dr Yasunari Kanda (National Institute of Health Sciences, Kawasaki, Japan) and Dr Benoît Maisonneuve (NETRI, Lyon, France)

• Bas ter Braak – In vitro prediction of drug-induced liver injury using toxprofiler
• Rajamuthu Srinivasan – Pragmatic Approach to inculcate New Approach Methodologies (NAMs) in Vaccine Safety Assessment
• Ludovico Buti – Evaluation of 3d human intestinal organoids as a platform for ev-a71 antiviral drug discovery (ESR presentation)
• Josephine Blersch – A human ex vivo model for assessing the immunotoxicity risk of engineered nanomaterials.

10:00-11:00:  Early Stage Researcher Session 3

Chairs: Dr Andreas Stucki (PETA Science Consortium International e.V., Stuttgart, Germany) and Dr Bambou Tan (L’Oréal, Paris, France)

• Luiz Ladeira – Physiological maps and their curation guidelines: paving the way for mechanistic NGRA
• Eliska Kuchovska – Ontology-based AI-driven innovative approach using DNT NAMs for NGRA
• Ruchir Shah – Artificial intelligence (AI)-driven morphological assessment of zebrafish embryo for developmental toxicity chemical screening
• Sara Sepehri – A next-generation risk assessment case study for the hair dye HC Yellow 13 focused on liver steatosis

11:30-12:45: Session 11a – In vitro methods for safety assessment of medical devices
Chairs: Helena Kandarova (CEM SAS, Bratislava, Slovakia) and Christian Pellevoisin (Urbilateria & Vitroscreen, France)

• Christian Pellevoisin – Challenges in regulatory acceptance of NAMs for medical devices
• Andy Forreryd – Regulatory approval of medical devices according to MDR using in vitro data from GARDskin Medical Device for skin sensitization assessment
• Tom Meseberg – Developement of a macrophage-based in vitro assay for alternative prediction of foreign body reaction to implantable medical devices
• Patrícia Gomes Ruivo – Reproductive Toxicity of vaginal lubricants: effects on oocyte maturation, fertilization and sperm viability

11:30 – 12:45: Session 11b – Cardiotoxicity and cardiac efficacy models
Chairs: Marketa Dvořáková (NIPH Prague, Praha, Czech Republic) and Andreas Stucki (PETA Science Consortium International e.V., Stuttgart, Germany)

• Georgia Papadimitriou – A weight of evidence approach for assessing cardiotoxicity in a regulatory context integrating New Approach Methodologies (NAMs)
• S. Rodrigues Ribeiro – SmartHeart – Novel cardiac-microtissues to answer the evolving challenges of drug discovery
• Laura-Sophie Frommelt – Green chemistry, red flags: ensuring cardiovascular safety by performing multiparametric analysis of phytochemicals using hIPSC-CMs-MEA assay.
• Niall Macquaide – Chronic Assessment of hERG Trafficking on Repolarisation and Cytotoxicity in iPSC Cardiomyocytes

14:00-19:00: Post-conference workshop with EPISKIN Academy

09:00-13:00: Post-conference workshop with EpiSkin Academy