Full Programme

Scientific Programme – Themes

Invited Speakers

• Bio-engineering, stem cells and disease models
• Models, biomarkers and assays for endocrine disruption and developmental toxicity
• Challenges in cosmetic regulation
• Models, biomarkers and assays for systemic and immune toxicity
• Organ-on-chip models
• Application of non-animal methods and concepts in food risk assessment (ILSI/ESTIV- session)
• Computational toxicology – in silico modelling, read-across, artificial intelligence and machine learning
• Local toxicity testing (safety and efficacy)
• Toxicokinetics and in vitro – in vivo extrapolation
• In vitro systems to assess respiratory toxicity
• In vitro methods for safety testing of biopharmaceuticals/biotherapies/vaccines
• Knowledge sharing and education
• Developmental Neurotoxicity (DNT)
• Implementation of NAMs into regulatory frameworks
• In vitro methods for safety assessment of medical devices
• In vitro COVID-19 research/lung and cardiovascular models
• Debate session: Building scientific confidence in NAMs Toward Regulatory Acceptance
• Poster teaser session
• Sponsored sessions
• Career development session
• JSAAE – sponsored session – Asian progress in 3Rs
• ILSI/ESTIV- sponsored lunch session

8:30-10:30: Session 2a – Models, biomarkers and assays for endocrine disruption and developmental toxicity
Chairs: Dr Thomas Knudsen (US EPA, Durham, USA) & Prof. Arno Gutleb (LIST, Luxembourg, Luxembourg)

• Thomas Knudsen – Computational systems models for human-predictive developmental toxicity (Invited lecture)
• Ingrid Langezaal – Upcoming validated mechanistic in vitro methods for the identification of thyroid disruptors.
• Andrew Tindall – Interlaboratory OECD Validation of the Rapid Androgen Disruption Adverse outcome Reporter (RADAR) assay
• Nina Hambruch – Validation of a New Approach Method (NAM) testing DIO1 inhibition using a human microsome-based, colorimetric assay
• Erica Buoso – Immunological implications of Endocrine Disrupting Chemicals (EDCs): RACK1 as a bridge between the endocrine and the immune systems
• Predrag Kukic – Beyond AOPs: A Mechanistic Evaluation of NAMs in DART Testing

8:30-10:30: Session 2b – Challenges in cosmetics safety

Chairs: Dr Nathalie Alépée (L’Oréal, Paris, France) & Dr Erwin Van Vliet (Houten, Utrecht, Netherlands)

• Jay Ingram – Global Regulatory Landscape for Cosmetics – Challenges and Opportunities in Utilising NAMs
• Els Adriaens – Overview of New Approach Methodologies for eye hazard identification according to UN GHS
• Nathalie Alépée – Application of an updated Next Generation Risk Assessment (NGRA) framework for skin allergy: a case study for Diethanolamine
• Véronique Poulsen – New challenges for environmental safety of cosmeticscccccccssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssss ssssssssssssssssssssssssssssssssssssssssssssssssssssssssss

11:00-13:00: Session 3a – Models, biomarkers and assays for systemic and immune toxicity
Chairs: Prof. Emanuela Corsini (Universita degli Studi di Milano, Milan, Italy) & Dr Jochem Louisse (Wageningen University, Wageningen, The Netherlands)

• Emanuela Corsini – New approach methodologies in immunotoxicology with a focus on immunosuppression (Invited lecture)
• Herve Groux – In vitro evaluation of both skin irritation and sensitization hazard of mixtures and finished products using the SENS-IS assay.
• Laura Suter-Dick – Multicellular, liver microtissues reliably depict key events of the liver fibrosis AOP
• Birgitte Lindeman – Immunotoxicity of crystalline silica particles: in vitro screening models and use of high-dimensional immune cell profiling
• Sabina Burla – A 3D Alveolar In Vitro Test System for the Prediction of Chemical Respiratory Sensitizers
• Andy Forreryd – In vitro assessment of skin sensitizing potential of process-related impurities in polymeric materials during product development.

11:00-13:00: Session 3b – Organ-on-a-chip & Microphysiology systems
Chairs: Dr Sofia Batista Leite (JRC, Ispra, Italy) & Dr Pau Sancho-Bru (Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain)

• Silvia Scaglione – A new immunocompetent OOC platform for culturing 3D human tissues with capillary flow-driven immune cells and investigating their cross-talk
• Julia Boos – Integration of human-stem-cell-based embryoid bodies into a microfluidic multi-tissue platform for systemic embryotoxicity testing
• Ilka Maschmeyer – Multi-Organ-Chip Technologies: The effect of application route on the metabolism
• Aude Rapet – A breathing lung-on-chip model: an advanced in-vitro tool for drug testing and toxicology
• Leopold Koenig – A microfluidic bone marrow chip for the safety profiling of complex large molecules in pre-clinical drug development
• Roberta Visone – A human beating heart on chip for online screening of drug cardiotoxicity

13:00-14:00: Lunch break, visiting exhibition area & poster viewing

13:00-14:00: ILSI/ESTIV- sponsored lunch session: Application of non-animal methods and concepts in food risk assessment
Chairs: Dr Isabelle Guelinckx (ILSI Europe, Belgium) & Dr Helena Kandarova (CEM SAS, Bratislava, Slovakia)

14:00-16:00: Session 4a – Computational toxicology – in silico modelling, read-across, artificial intelligence and machine learning
Chairs: Prof. Olivier Taboureau (University Paris Diderot, Paris, France) & Dr Tim Allen (Ladder Therapeutics, Toronto, Canada)

• Olivier Taboureau – Bioinformatics and Network science applied in toxicology (Invited lecture)
• Thomas Hartung – The key role of computational toxicology for the 21^st Century 2.0 (Invited lecture)
• Ignacio Tripodi – Turning AOPs into testable hypotheses with natural language processing and tissue-specific knowledge graphs
• Prachi Pradeep – Integrating in vitro and in silico data for screening and characterization of tattoo pigments for human health risk assessment
• Donatella Carpi – Modelling in vitro time-concentration response of gene expression to chemical exposure

14:00-16:00: Session 4b – Local toxicity testing (safety and efficacy)
Chairs: Dr Montserrat Mitjans (Universitat de Barcelona, Barcelona, Spain) & Dr Helena Kandarova (CEM SAS, Bratislava, Slovakia)

• Els Adriaens – A new OECD guideline based on Defined Approaches for Eye Hazard Assessment of non-surfactant liquids combining Physicochemical Properties and OECD-approved Test Methods
• Manon Barthe – Modeling skin inflammation using 2D and 3D in vitro models
• Elena Grasselli – In vitro investigation of aluminum bioavailability after exposure of oral care products
• Eric Andres – Integrative evaluation strategy for skin sensitization : the mixtures challenge
• Renato Ivan de Ávila – Application of a next-generation risk assessment framework for skin sensitisation using new approach methodologies (NAMs): geraniol case study

16:30-17:30: Early Stage Researcher Session 1
Chairs: Dr Erwin van Vliet (Houten, Utrecht, Netherlands) & Dr Pau Sancho-Bru (Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain)

• Méryl Roudaut – A single procedure to generate functional hiPSCs-derived liver organoids – Towards an innovative tools suitable for drug screening
• Jördis Klose – In vitro screening for developmental neurotoxicity of flame retardants using a 3D human primary neural progenitor cell assay: a case study
• Yanying Ma – Predicting developmental toxicity of pyrethroid insecticides in vitro using human-induced pluripotent stem cells
• Rima Souki – Characterization of extracellular vesicles released by peripheral blood mononuclear cells exposed to polycyclic aromatic hydrocarbons and study of their interaction with endothelial cells
• Michelle Jäschke – Towards a personalized kidney-on-a-chip derived from induced pluripotent stem cells

17:30-18:30: Early Stage Researcher Session 2
Chairs: Dr Montserrat Mitjans (Universitat de Barcelona, Barcelona, Spain) & Dr. Marketa Dvořáková (NIPH Prague, Prague, Czech republic)

• Heart Avner Ehrlich – The Electro-Mitochondrial Coupling of a Microphysiological Human
• Ahmet Kati – Affinity prediction between cosmetic ingredients and CD54/CD86 receptors by molecular docking: A novel approach to predict skin sensitisation potential
• Filipa Leal – Electrospun scaffold-based approaches for toxicological screening in skin, pulmonary, and intestinal epithelial tissue
• Zachary Sitte – Developing a method to incorporate laminar flow into scaffold-based 3D cell cultures for more physiologically relevant nutrient and drug delivery.
• Marie Frerejacques – Kidney organoids for toxicology: application to the development of Adverse Outcome Pathway (AOP) study of nephrotoxicity induced by uranium

16:30-18:30: Sponsored session
Chairs: Erin Hill (IIVS, Gaithersburg, MD, USA) & Dr Clive Roper (Roper Toxcilogy Consulting Limited, UK)

• Angela Hvitved – Research support to replace animal models: the Alternatives Research & Development Foundation (ARDF) Annual Open grant program

• Salvatore Simmini – Organoids and their application in toxicity studies

• Caio Valeriano – Technologies Bioprinting ‐ a paradigm shift in the field of toxicology testing

08:30-10:30: Session 5a – Toxicokinetics and in vitro–in vivo extrapolation
Chairs: Dr Nicole Kleinstreuer (NICEATM, Durham, USA) & Dr Jochem Louisse (Wageningen University, Wageningen, The Netherlands)

• Nicole Kleinstreuer – An Open, Accessible Interface for Contextualizing Chemical Effects (Invited lecture)
• Andreas Schepky – Crucial role of Toxicokinetics and ADME methods in the application of Next-generation Risk Assessment
• Ans Punt – Generic PBK models based on in vitro and in silico input data: How to gain trust in the results?
• Sophie Werner – Use of a 3d-in vitro model for the assessment of liver metabolism related to neurotoxicity of occupationally relevant chemicals
• Norman Nowak – Design and Application of a Physiologically Based Kinetic (PBK) Model for Uptake of Gases and Vapours
• Julia Huchthausen – High-Throughput Assessment of the Abiotic Stability of Test Chemicals in In Vitro Bioassays

08:30-10:30: Session 5b – In vitro systems to assess respiratory toxicity
Chairs: Erin Hill (IIVS, Gaithersburg, MD, USA) & Dr Clive Roper (Roper Toxcilogy Consulting Limited, UK)

• Andreas Stucki – Evaluating human cell-based in vitro systems to assess respiratory toxicity – is more complex better?
• Artur Christian Garcia da Silva – Development and characterization of a decellularized bronchial scaffold for application in physiologically relevant 3D airway models reconstruction
• Victoria Hutter – The application of alveolar macrophage responses for respiratory safety assessment
• Jan Markus – Prediction of acute respiratory toxicity of volatile liquids using physiologically relevant in vitro inhalation model
• Margaux Cochard – Impact of airborne particulate matter from port, industrial and urban areas on oxidative and inflammatory responses on lung cells.

11:00-13:00: Session 6a – In vitro methods for safety testing of biopharmaceuticals/biotherapies/vaccines
Chairs: Dr Michael Rothe (Hannover Medical School, Hannover, Germany) & Prof. Anne Marie Vinggaard (Technical University of Denmark, Denmark)

• Michael Rothe – In vitro assays to predict retroviral vector-induced genotoxicity (Invited lecture)
• Jean-Pierre Valentin – State-of-the-Art in Secondary Pharmacology and its Impact on the Safety of New Medicines
• Terry Riss – Exploiting Real-Time Assays to Multiplex Orthogonal Methods and Determine Mechanism of Action During In Vitro Safety Testing
• Bettina Seeger – Botulinum neurotoxin potency testing in vitro – Motor neurons differentiated from human-induced pluripotent stem cells serve as highly suitable target cells
• Zachary Sitte – Supported Gel Slabs: Decreasing the barrier of entry for use of 3D cell cultures in the drug discovery workflow
• Jean-Pierre Valentin – Mechanistic investigations and early de-risking of QRS complex widening to improve cardiac safety profile of novel anti-malarials

11:00-13:00: Session 6b – Knowledge sharing and education
Chairs: Prof. Pilar Vinardell (Universitat Barcelona, Barcelona, Spain) & Erin Hill (IIVS, Gaithersburg, MD, USA)

• Pierre Deceuninck – The science of sharing knowledge about models and methods
• João Barroso – Scientific Validity of Non-Animal-Derived Antibodies
• Tilo Weber -Animals in the (Petri) Dish: Towards a Truly Animal-free Laboratory
• Catharine Krebs – Outcomes of a workshop to address animal methods bias in scientific publishing
• Renato de-Avila – A Course on Animal-Free Risk Assessment of Cosmetics and Cosmetic Ingredients

13:00-14:00: Lunch break, visiting exhibition area & poster viewing

13:00-14:00: JSAAE – sponsored session – Asian progress in 3Rs:
3rd Asian congress and KSAAE updates
Chairs: Dr Yasunari Kanda (National Institute of Health Sciences, Japan) & Dr Kwang-Mahn Kim (Yonsei University, South Korea)

08:30-10:30: Session 7a – Developmental Neurotoxicity (DNT)
Chairs: Dr. Ellen Fritsche & Clive Roper (Roper Toxicology Consulting Limited, UK)

• Ellen Fritsche – Developmental neurotoxicity (DNT) as a case study on how to assemble an in vitro testing battery for a complex regulatory in vivo endpoint
• Eliska Kuchovska – First steps for creating an ontology for cognitive function defects for regulatory application
• Maxime Culot – Evaluation of a human iPSC-derived BBB model for repeated dose toxicity testing
• Elisabet Teixido – Comparative developmental neurotoxicity of NSAIDs in the zebrafish
• Marta Barenys – an adverse outcome pathway (AOP) based approach to identify changes in prenatal brain programming
• Laure-Alix Clerbaux – effects of spike protein and toxin-like peptides found in covid-19 patients on human 3d neuronal/glial model undergoing differentiation: possible sars-cov-2 implications for brain development

08:30-10:30: Session 7b – Implementation of NAMs into regulatory frameworks – establishing scientific confidence, development of standards and good practices
Chairs: Dr. Marketa Dvořáková (NIPH Prague, Prague, Czech republic) & Dr. Jochem Louisse (Wageningen University, Wageningen, The Netherlands)

• Sandra Coecke – Method developers are key players ensuring in-house reproducibility and relevance of in vitro methods paving the way for regulatory acceptance
• Amanda Ulrey – Good In Vitro Method Practices Certification: A Roadmap for Implementation and Harmonization
• Annelies Noorlander – towards standardization of testing methods for comparative in vitro metabolism (CIVM) studies
• Manon Beekhuijzen – IMPACT OF CURRENT REGULATORY CHANGES ON TOXICOLOGY TESTING REQUIREMENTS FOR CHEMICALS IN EUROPE

11:00-12:40: Session 8a – In vitro methods for safety assessment of medical devices
Chairs: Dr. Christian Pellevoisin (MatTek Life Sciences, France) & Dr. Helena Kandarova (CEM SAS, Bratislava, Slovakia)

• Christian Pellevoisin – ISO 10993 SERIES: Added value of 3D models for Biocompatibility testing of medical devices (Invited lecture)
• Stefan Weissensteiner – Why in-vivo methods for the assessment of sensitization might be outdated in the near future
• Robert Teixido – Hemocompatibility of bacteriophobic coated catheters
• Marisa Meloni – Biocompatibility of medical devices: eye irritation of preserved and unpreserved eye drops on HCE model
• Hervé Groux – Measurement of metal sensitizer potency with the SENS-IS assay. Application to Medical devices

11:00-12:40: Session 8b – In vitro COVID-19 research / Lung and cardiovascular models
Chairs: Prof. Arno Gutleb (LIST, Luxembourg, Luxembourg) & Dr. Yasunari Kanda (National Institute of Health Sciences, Japan)

• Sandra Coecke – Knowledge from Human Relevant Cell, Tissue and Mathematics-based Methods as Key Tools for Understanding COVID-19 Dynamics, Kinetics, Symptoms, Risk Factors and Non-conventional Treatments
• Samuel Constant – 3D Human airway epithelial models to study SARS-CoV-2 pathogenesis and to discover antivirals
• Axelle Cooreman – Effects of drugs formerly repurposed for COVID-19 treatment on connexin43 hemichannels and pannexin1 channels
• Chloé Chivé – Use of a human bronchial epithelium model to assess the impact of PM2.5 exposure on the severity of viral infections
• Avner Ehrlich – Metabolic Regulation of SARS-CoV-2 Infection

14:00-19:00: Post-conference workshop with EPISKIN Academy

14:00-19:00: ASPIS Cluster meeting

08:30-16:00: Post-conference workshop with EpiSkin Academy

08:30-16:00: ASPIS Cluster meeting